THE COMPLETE NUCLEOTIDE-SEQUENCE OF TURNIP MOSAIC-VIRUS RNA JAPANESE STRAIN - BRIEF REPORT

The complete nucleotide sequence of the RNA genome of turnip mosaic vi rus Japanese strain (TuMV-J) has been determined from five overlapping cDNA clones and by direct sequencing of viral RNA. The RNA sequence w as 9833 nucleotides in length, excluding a 3' terminal poly(A) tail. A n AUG triplet at position 130-132 was assigned as the initiation codon for the translation of the genome size viral polyprotein which would consist of 3 164 amino acid residues. Interestingly, a different;amino acid sequence (continuous twenty amino acids) within the cytoplasmic inclusion protein between TuMV-J and Canadian strain of TuMV was obser ved, caused by an insertion and a deletion of nucleotides.