IMPROVED METHOD FOR STUDY OF CHROMOSOMES OF CHORIONIC VILLUS SAMPLES

Authors
CHOWDHURY MR MATHUR R PRABHAKARA K SINGH G DUBEY S VERMA IC
Citation
Mr. Chowdhury et al., IMPROVED METHOD FOR STUDY OF CHROMOSOMES OF CHORIONIC VILLUS SAMPLES, INDIAN JOURNAL OF MEDICAL RESEARCH, 104, 1996, pp. 269-272
Citations number
11
Categorie Soggetti
Medicine, General & Internal",Immunology
Journal title
INDIAN JOURNAL OF MEDICAL RESEARCH → ACNP
ISSN journal
09715916
Volume
104
Year of publication
1996
Pages
269 - 272
Database
ISI
SICI code
0971-5916(1996)104:<269:IMFSOC>2.0.ZU;2-L
Abstract
Chorionic villus sampling (CVS) offers rapid prenatal diagnosis of chr omosomal disorders. We evaluated four methods for chromosomal analysis of chorionic villi (three based on direct preparations and one on lon g-term culture) in order to define a method which would provide good q uality metaphases in sufficient numbers. The direct culturing method u sing synchronisation and dissociation with dispase was as good as long -term culturing. The optimal period of gestation for study was 10-12 w k.