APPLICATION OF LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRY IN NATURAL PRODUCT ANALYSIS - EVALUATION AND OPTIMIZATION OF ELECTROSPRAY AND HEATED NEBULIZER INTERFACES
Sl. Zhou et M. Hamburger, APPLICATION OF LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRY IN NATURAL PRODUCT ANALYSIS - EVALUATION AND OPTIMIZATION OF ELECTROSPRAY AND HEATED NEBULIZER INTERFACES, Journal of chromatography, 755(2), 1996, pp. 189-204
Citations number
48
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A representative set of 46 secondary metabolites of plant and microbia
l origin was examined with electrospray and atmospheric pressure chemi
cal ionization MS in both positive and negative ion mode, in order to
evaluate the applicability of atmospheric pressure ionization LC-MS as
a tool for rapid identification and dereplication of bioactive compou
nds in the drug screening process. MS characteristics of different com
pound classes were determined on the basis of quasi-molecular ion and
other adduct ion responses observed with the two LC-MS interfaces. Int
erface parameters and LC mobile phase composition were optimized and a
n analytical protocol applicable to a wide range of secondary metaboli
tes is proposed.