ONLINE PRECONCENTRATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBON-DNA ADDUCTS USING COPPER PHTHALOCYANINE TRISULFONIC ACID AS A GROUP-SPECIFIC ADSORBENT

An on-line preconcentration high-performance liquid chromatography sys tem for the determination of polycyclic aromatic hydrocarbon-DNA adduc ts such as trihydroxytetrahydrobenzo[a]pyrene-deoxy in a DNA hydrolysa te is described. Restricted-access copper phthalocyanine (CP) diol mod ified silica was used as a group-specific sorbent. For the determinati on of the benzo[a]pyrene adduct, two preconcentration columns (precolu mns) in a dual-precolumn setup were used. Elution of the benzo[a]pyren e adduct from the CP precolumn was performed with 100% methanol. The a nalyte was re-concentrated on a C-18 precolumn applying on-line diluti on with water. The C-18 precolumn can be desorbed with the LC mobile p hase used for the analytical column. Trihydroxytetrahydrobenzo[a]pyren e-deoxy was determined in a DNA hydrolysate using the dual-precolumn p rocedure. A recovery of 95% was achieved. With fluorescence detection, a mass detection limit of 0.1 pmol injection was obtained.