INDUCTION OF SULFATED GLYCOPROTEIN-2 (CLUSTERIN) AND GLIAL FIBRILLARYACIDIC PROTEIN (GFAP) RNA EXPRESSION FOLLOWING TRANSIENT GLOBAL-ISCHEMIA IS DIFFERENTIALLY ATTENUATED BY LY231617

Sulfated glycoprotein-2 (SGP-2) is a secreted glycoprotein that along with GFAP has emerged as a prominent molecular marker of neurodegenera tion. In the present study, we have evaluated further the relationship between SGP-2, GFAP and neurodegeneration, by examining the effects o f LY231617, a potent antioxidant, on expression of SGP-2 and GFAP foll owing four vessel occlusion (4VO). GFAP and SGP-2 RNA levels increased several fold in hippocampus and caudate nucleus in response to 30 min of 4VO. LY231617 treatment markedly attenuated the induction of GFAP RNA in both hippocampus and caudate nucleus, consistent with the signi ficant neuroprotection observed histologically. In contrast, LY231617 treatment blunted SGP-2 RNA expression only in the hippocampus; SGP-2 RNA expression in caudate nucleus was similar to vehicle-treated 4VO, despite the marked attenuation of neuronal damage in both areas by LY2 31617. These data suggest region-specific differential regulation of S GP-2 and GFAP RNA induction.