STAPLABIN, A NOVEL FUNGAL TRIPRENYL PHENOL WHICH STIMULATES THE BINDING OF PLASMINOGEN TO FIBRIN AND U937 CELLS

A novel triprenyl phenol, designated staplabin, has been isolated from a culture of Stachybotrys microspora IFO 30018 by solvent extraction and successive chromatographic fractionation using silica gel, Sephade x LH-20 and silica ODS columns. By a combination of spectroscopic anal yses, the structure of staplabin is proposed to be o-6H-pyrano[2,3-e][ 1,3]dihydroisoindolyl)pentanoic acid. Staplabin stimulated the binding of plasminogen, the zymogen of the Fibrinolytic serine protease plasm in, to both fibrin and U937 cells. Binding was elevated 2-fold at a co ncentration of 0.3 similar to 0.5 mM.