A RELIABLE AND SENSITIVE ENZYME-IMMUNOASSAY METHOD FOR MEASURING 8-ISOPROSTAGLANDIN F2-ALPHA - A MARKER FOR LIPID-PEROXIDATION AFTER EXPERIMENTAL BRAIN INJURY

Authors
HOFFMAN SW ROOF RL STEIN DG
Citation
Sw. Hoffman et al., A RELIABLE AND SENSITIVE ENZYME-IMMUNOASSAY METHOD FOR MEASURING 8-ISOPROSTAGLANDIN F2-ALPHA - A MARKER FOR LIPID-PEROXIDATION AFTER EXPERIMENTAL BRAIN INJURY, Journal of neuroscience methods, 68(2), 1996, pp. 133-136
Citations number
24
Categorie Soggetti
Neurosciences
Journal title
Journal of neuroscience methods → ACNP
ISSN journal
01650270
Volume
68
Issue
2
Year of publication
1996
Pages
133 - 136
Database
ISI
SICI code
0165-0270(1996)68:2<133:ARASEM>2.0.ZU;2-R
Abstract
The objectives of this study were to determine (1) if levels of 8-isop rostaglandin F-2 alpha (8-isoPGF(2 alpha)), a non-enzymatically produc ed prostaglandin, were increased after cortical contusion and (2) if e nzyme immunoassay (EIA) could be used to quantify 8-isoPGF(2 alpha) le vels. 24 h after the contusion there was a significant rise in 8-isoPG F(2 alpha) compared to control levels. The levels returned to baseline by 72 h postinjury. The results show that this EIA method is a reliab le and sensitive technique for measuring brain lipid peroxidation.