Gf. Khan et W. Wernet, PLATINIZATION OF SHAPABLE ELECTROCONDUCTIVE POLYMER FILM FOR AN IMPROVED GLUCOSE SENSOR, Journal of the Electrochemical Society, 143(10), 1996, pp. 3336-3342
This paper describes a novel electrode material for the preparation of
a first generation amperometric biosensor. The material consists of a
flexible conductive polymer film of polypyrrole doped with polyanions
and a layer of microporous Pt black, prepared electrochemically on th
e polymer film. Sensors fabricated with this material produce a compar
atively higher H2O2 oxidation current at a lower applied potential. Gl
ucose sensors were prepared by adsorbing glucose oxi dase at the porou
s Pt black structure, covering with gelatin, and finally cross-linking
with glutaraldehyde at dry condition. The developed sensors showed si
gnificantly improved performance over similar reported sensor systems.
The performance of the glucose sensor was evaluated by a specially de
signed flow injection analysis (FIA) system. The sensors were continuo
usly polarized at 25 degrees C and glucose samples were automatically
injected at 30 min intervals. The sensors worked at 0.3 to 0.4 V and p
roduced a huge current response (>1 mA/cm(2)) with a wide linear range
of detection (0 to 100 mM). The system effectively recycles oxygen, t
hus, the response current was not affected by a variation of oxygen co
ncentration of the buffer. The interference of ascorbic acid, uric aci
d, bilirubin, etc. (at a physiological level) produced a current withi
n the experimental error level. The sensor showed an extended working
and shelf life.