O. Ishihara et al., PROSTAGLANDIN E(2) PRODUCTION BY ENDOGENOUS SECRETION OF INTERLEUKIN-1 IN DECIDUAL CELLS OBTAINED BEFORE AND AFTER THE LABOR, Prostaglandins, 52(3), 1996, pp. 199-208
Previous reports revealed that interleukin-1(IL-1) was involved in the
process of premature labor in the Gases with intrauterine infection.
However, the roles of the cytokine in normal spontaneous labor remain
uncertain. The present studies aimed at determining the involvement of
the cytokine in prostaglandin (PG)E(2) production during labor by the
third trimester decidual cells. The cells were obtained at the time o
f normal spontaneous delivery (NVD) and elective cesarean section (ECS
). The NVD cells produced significantly more amount of PGE2 than the E
CS cells and the both cells responded to the addition of IL-1 beta to
increase PGE(2) production. A specific inhibitor of cyclooxygenase-2 (
COX-2), NS398, decreased basal PGE(2) production and inhibited the sti
mulatory effect of IL-1 beta in a dose dependent manner in NVD cells.
The NVD cells secreted more amount of IL-1 beta than the ECS cells and
contained more amount of preprocessed 31kD IL-1 beta inside the cells
. The addition of recombinant soluble human IL-1 receptor (type I) not
only blocked the effect of IL-1 beta on PG secretion, but significant
ly reduced the basal production of PGE2 by NVD cells. These results in
dicate that decidual PG production depends upon COX-2 after the onset
of labor. Besides it seems likely that endogenously produced IL-1 beta
may be involved in autocrine or paracrine fashion in inducing COX-2 a
fter the onset of labor.