S. Rea et al., POSSIBILITY OF USING SINGLE-STRAND CONFORMATION POLYMORPHISM (SSCP) ANALYSIS FOR DISCRIMINATING EUROPEAN PIG AND WILD BOAR MEAT SAMPLES, Italian journal of food sciences, 8(3), 1996, pp. 211-220
For discriminating domestic pig from European wild boar meat and meat
products, a particular portion of mitochondrial DNA (D-loop region), c
onsidered as the site of significant mutations in the nucleotidic sequ
ence, was amplified by Polymerase Chain Reaction (PCR) and used in Sin
gle Strand Conformation Polymorphism (SSCP) analysis. The comparison d
id not allow the direct discrimination of pig and wild boar samples. N
evertheless, the sequencing analyses carried out on the DNA fragments
of wild boar samples that showed different SSCP patterns, indicated th
at there are preferential sites of mutation, not yet reported in Europ
ean wild boar. All samples carrying the substitution T/C in position 2
74 gave a single SSCP pattern, different from the others, suggesting a
probable specific mutation. However, we consider that the calculation
of the frequency of each substitution in the two animals could be use
ful for developing a method for discriminating pig from wild boar meat
and meat products.