PREIMPLANTATION EMBRYO CHROMOSOME ANALYSIS BY PRIMED IN-SITU LABELINGMETHOD

Objective: To present the use of primed in situ labeling method in pre implantation diagnosis. Design: Double- and triple-primed in situ labe ling were performed on 10 morphologically abnormal preimplantation emb ryos, using combinations of specific primers for chromosomes 9, 13, 16 , 18, 21, X, and Y. Setting: Embryos were obtained from patients at th e Montpellier University Hospital. Patient(s): Seven women undergoing IVF at the Montpellier University Hospital.Intervention(s): Isolated i nterphase nuclei from poor quality preimplantation embryos were prepar ed for primed in situ labeling technique. Main Outcome Measure(s): Num erical abnormalities assessed by primed in situ labeling analysis. Res ult(s): Using directly fluorescent-labeled nucleotides, the labeling r eaction for three chromosomes did not exceed 2.30 hours. Only three an alyzed embryos appeared to be chromosomally normal. Mosaicism, aneupoi dy, and haploidy were observed in the seven other embryos. Conclusion( s): The primed in situ labeling method offers a simple and reliable sc reening tool for gender determination and aneuploidy detection. The us e of this technique may contribute to significantly improve the proced ure of preimplantation diagnosis.