THE GENOME ORGANIZATION AND AFFINITIES OF AN AUSTRALIAN ISOLATE OF CARROT MOTTLE UMBRAVIRUS

Citation
Mj. Gibbs et al., THE GENOME ORGANIZATION AND AFFINITIES OF AN AUSTRALIAN ISOLATE OF CARROT MOTTLE UMBRAVIRUS, Virology, 224(1), 1996, pp. 310-313
Citations number
44
Categorie Soggetti
Virology
Journal title
ISSN journal
00426822
Volume
224
Issue
1
Year of publication
1996
Pages
310 - 313
Database
ISI
SICI code
0042-6822(1996)224:1<310:TGOAAO>2.0.ZU;2-Y
Abstract
The genomic sequence of an Australian isolate of carrot mottle umbravi rus (CMoV-A) was determined from cDNA generated from dsRNA. This provi des the first data on the genome organization and phylogeny of an umbr avirus. The 4201-nucleotide genome contains four major open reading fr ames (ORFs). Analysis suggests that ORF2 encodes an RNA-dependent RNA polymerase, that ORF4 encodes a movement protein, and that the virus h as no coat protein gene; The functions of ORFs 1 and 3 remain unknown. ORF2 is probably translated following ribosomal frameshifting. ORFs 3 and 4 are probably translated from a subgenomic mRNA. Sequence compar isons showed CMoV-A to be closely related to pea enation mosaic RNA2 ( PEMV-RNA2), but also to have affinities with the Bromoviridae. These f indings shed light on the relationships between the luteoviruses, PEMV , and the umbraviruses and on the relationships between the carmo-like viruses and the Bromoviridae. (C) 1996 Academic Press, Inc.