The presence of cholesterol, 7-dehydrocholesterol, cholecalciferol (vi
tamin D-3) and its hydroxylated metabolites, 25-hydroxycholecalciferol
(25-OH D-3) and 1 alpha,25-dihydroxycholecalciferol (1 alpha,25-diOH
D-3), was demonstrated in cell cultures derived from sterile leaves of
Solanum malacoxylon. When calli were grown in the dark, 7-dehydrochol
esterol was found and identified on the basis of its chromatographic a
nd spectroscopic properties. The analysis of calli grown in the light
revealed the presence of vitamin D-3 and 25-OH D-3, where 7-dehydrocho
lesterol was not detected. In suspended cells, maintained in the light
, vitamin D-3, 25-OH D-3 and 1 alpha,25-diOH D-3 were found, but no 7-
dehydrocholesterol was detected. Our results suggest that 7-dehydrocho
lesterol may arise as an intermediate in the biosynthesis of vitamin D
-3 in S. malaconylon. A photoreaction from 7-dehydrocholesterol may be
operative in the biosynthesis of cholecalciferol, thus suggesting tha
t in this plant there is a similar pathway to that in animals. Copyrig
ht (C) 1996 Elsevier Science Ltd