EFFECT OF PERFUSION RATE ON THE TIME-COURSE OF INSULIN-MEDIATED SKELETAL-MUSCLE GLUCOSE-UPTAKE

To better define the time course of skeletal muscle glucose uptake and its modulation by changes in perfusion, we performed systemic euglyce mic-hyperinsulinemic clamps (40 mu . m(-2) . min(-1)) for a 90-min per iod in a group of lean, insulin-sensitive subjects (n = 9) on two occa sions (similar to 4 wk apart) with insulin-mediated vasodilation intac t or inhibited. Insulin-mediated vasodilation was inhibited by an intr afemoral artery infusion of N-G-monomethyl-L-arginine (L-NMMA), a spec ific inhibitor of nitric oxide synthase. During the study, leg blood f low (LBF) and arteriovenous glucose difference (AVG Delta) were measur ed every 10 min; leg glucose uptake (LGU) was calculated as LGU = LBF x AVG Delta. The systemic insulin infusion caused a time-dependent inc rease in LBF from 0.194 +/- 0.024 to 0.349 +/- 0.046 l/min (P < 0.01). The intrafemoral artery infusion of L-NMMA completely inhibited this increase in LBF. AVG Delta, LGU, and whole body glucose disposal rates increased in a time-dependent manner in both studies. The maximum AVG Delta was lower with insulin-mediated vasodilation intact than when i nhibited (25.9 +/- 2.5 vs. 35.0 +/- 1.6 mg/dl, P < 0.001). The time to achieve half-maximal (T-1/2) AVG Delta was somewhat longer with insul in-mediated vasodilation intact compared with inhibited (35.6 +/- 4.1 vs. 29.7 +/- 1.6 min, P < 0.01). Maximal LGU was 93.9 +/- 26.8 and 57. 2 +/- 11.6 mg/min (P < 0.005), and the T-1/2 LGU was 50.2 +/- 16.0 and 36.3 +/- 8.8 min (P = 0.1) during intact and inhibited insulin-mediat ed vasodilation, respectively. Thus insulin-mediated vasodilation has a modest effect in slowing the time course at which insulin stimulates glucose uptake but has a marked effect in augmenting the maximal rate of insulin-stimulated glucose uptake in skeletal muscle. Impaired ins ulin-mediated vasodilation, as observed in patients with essential hyp ertension, may explain, at least in part, the insulin resistance obser ved in these patients.