POROUS POLYSULFONE COATED WITH PLATELET-DERIVED GROWTH FACTOR-BB STIMULATES PROLIFERATION OF HUMAN PERIODONTAL-LIGAMENT FIBROBLASTS

The purpose of this study was to investigate if the treatment of porou s polysulfone (PPSF) with various concentrations of platelet-derived g rowth factor-BB (PDGF-EB) would stimulate the proliferation of the adh erent human periodontal ligament fibroblasts (HPDLF) in culture. Steri lized PPSF cylinders were immersed in an Eagle's minimum essential med ium supplemented with 0.5% fetal bovine serum and 1% penicillin/strept omycin containing either 0, 10, 20, or 50 ng/ml of PDGF-BB for 24 hour s. After 24 hours, the PPSF cylinders were removed and allowed to dry then placed in culture plates for each time point. Pooled HPDLF (8 x 1 0(4)) and H-3-thymidine in medium were pipetted into each well to cove r the treated and control PPSF cylinders and plates were then incubate d. At 1, 4, and 10 days the PPSF cylinders were removed and macromolec ular precipitation was performed. Incorporation of H-3-thymidine was m easured and a 2-way ANOVA with repeated measures was performed. In add ition, determination of binding and release was performed using I-125- PDGF-BB treated PPSF at 0, 2, 12, and 24 hours, and at 4 and 10 days. Results showed that the effects on HPDLF were significant for dose (P = 0.0012; F = 5.74) and time (P = 0.0001; F = 40.83). At 4 days, the p ercent increases above the control for the doses 10, 20, and 50 ng/ml were 192%, 310%, and 162% respectively. In conclusion, our findings su ggest that treating PPSF with PDGF-BB results in a significant increas e in the proliferation of HPDLF cells adherent to PPSF.