GLUCOSAMINYLMURAMYLDIPEPTIDE-INDUCED CHANGES IN PHENOTYPE OF MELANOMA-CELLS RESULT IN THEIR INCREASED LYSIS BY PERIPHERAL-BLOOD CELLS

Citation
T. Valyakina et al., GLUCOSAMINYLMURAMYLDIPEPTIDE-INDUCED CHANGES IN PHENOTYPE OF MELANOMA-CELLS RESULT IN THEIR INCREASED LYSIS BY PERIPHERAL-BLOOD CELLS, International journal of oncology, 9(5), 1996, pp. 885-891
Citations number
35
Categorie Soggetti
Oncology
ISSN journal
10196439
Volume
9
Issue
5
Year of publication
1996
Pages
885 - 891
Database
ISI
SICI code
1019-6439(1996)9:5<885:GCIPOM>2.0.ZU;2-6
Abstract
Flow cytometry was used to show that biologically active N-acetylgluco samine-containing muramylpeptides (GMPs) induced in vitro dose-depende nt increase in the expression of tumor-associated antigens (TAAs) char acteristic for colon and mammary gland carcinomas, melanoma and lung a denocarcinoma. Forty to two hundred percent enhancement in TAA-express ing cells was observed after 18-48 h incubation with GMPs. In contrast , MHC class I antigen expression was not altered. Using MTT and chromi um-release assays, melanoma cells treated in vitro with GMDP were show n to be more susceptible to killing by peripheral blood cells of healt hy donors than non-treated cells. Fractionation of blood cells reveale d that platelets were responsible for this effect.