DEVELOPMENT OF A DUAL-CHANNEL CHEMILUMINESCENCE FIA SYSTEM FOR RAPID MEASUREMENT OF FISH FRESHNESS

A dual channel chemiluminescence FIA system for the rapid measurement of the fish freshness index KI {KI = (([HxR] + [Hx])/([IMP] + [HxR]+ [ Hx])) x 100} where [IMP], [HxR] and [Hx] are, respectively, the inosin e 5'-monophosphate, inosine and hypoxanthine contents of fish meat was developed. The system consisted of a pair of chemiluminescence FLAs ( CL-FIA); one for the measurement of [IMP] + [HxR] + [Hx], and the othe r for [HxR] + [Hx]. IMP, HxR or Hx were measured using a reactor compr ising immobilized alkaline phosphatase, purine nucleoside phosphorylas e (PNP) and xanthine oxidase (XOD). The calibration curves for IMP, Hx R or Hx were linear from 0.06 mu M to 100 mu M for a sample volume of 20 mu l. The time for a singe KI measurement was less than 1 min. This fish freshness FIA system is rapid, convenient and applicable for fis h freshness measurements in real fish samples.