T. Lindfors et al., A JET RING CELL WITH A RENEWABLE SOLID SUPPORT FOR AMPEROMETRIC DETECTION OF GLUCOSE IN A SEQUENTIAL INJECTION-ANALYSIS SYSTEM, Analytical letters, 29(13), 1996, pp. 2257-2267
A new amperometric method for the detection of glucose has been develo
ped. A jet ring cell with a renewable solid support (JRR) is connected
to a sequential injection analysis (SIA) system. The solid support, c
onsisting of small spherical particles of agarose gel, with a mean bea
d size of 90 mu m, is coupled with the enzyme glucose oxidase (GOD). T
he suspension is injected through a multiport valve in the SIA-system
and is trapped in the JRR cell by a gap of 10 mu m between the glassy
carbon (GC) working electrode and a stainless steel counter electrode.
The subsequent injection of glucose results in formation of hydrogen
peroxide, which is detected by oxidation at 1.0 V (vs. Ag/AgCl/KCl (3
M)), Thereafter the suspension is removed by reversing the pump flow a
nd subsequently replaced with fresh suspension for the next determinat
ion of glucose, The Linear working range for the JRR-sensor, at this s
tage of the work, is 100 mu M - 5 mM.