PURIFICATION AND STRUCTURAL-ANALYSIS OF S UBSTANCES IN WASABI (EUTREMA-WASABI MAXIM) THAT SUPPRESS THE GROWTH OF MKN-28 HUMAN STOMACH-CANCER CELLS

The objective of this paper is to identify growth-suppressing substanc es in wasabi on MKN-28 human stomach cancer cells. A water extract fra ction (<molecular weight 5000) of wasabi suppressed the growth of MKN- 28 derived from human stomach cells, The growth inhibitting principles were purified from the extract and analyzed, The active fractions wer e isolated by repeated column chromatography on gel filtration and rev erse-phase HPLC. The pi fraction, one of the fractions separated by Se phadex G-15 gel filtration, showed the most marked suppressive effect on MKN-28 among the fractions. The P-5 fraction suppressed the growth of MKN-28 stronger than the antitumor agents such as 5-fluorouracil an d Mitomycin C, On the final step of isolation, the sample that had sho wed the suppressive effect was divided into four fractions by the reve rse phase HPLC. Structural analysis was performed using the fraction ( retention time=12 min) with the greatest suppressing activity. Examina tion by FAB-MS and EI-MS yielded a molecular weight oi 205 with a chem ical composition of C-8, H-15, N-1, O-1, S-2. By using IR and NMR, we have identified 6-metylsulfinylhexyl isothiocyanate as one of the supp ressing components on the growth of MKN-28 in wasabi.