In this paper, ire studied tile mechanism of the increase of firmness
in strawberry fruit treated with 100% CO2. Internal CO2 concentration
in treated fruit increased immediately after the treatment and maintai
ned the high level during treatment. But, ii decreased rapidly after r
elease of the treatment and reached the same level of untreated ones.
This treatment rapidly decreased the ratio of water soluble pectin con
tent against total pectin content and increased the ratio of hexa meta
phosphate soluble pectin content during and after the treatment, hut d
id not affect the ratio of hydrochloric acid soluble pectin to total p
ectin in the fruit, Pectin methyl esterase activity was higher in trea
ted fruit than the one in untreated fruit during and after the treatme
nt. The ratio of Cn content of NaCl-soluble fraction (contained pectin
substances) to total Ca content was higher in the treated fruit durin
g and after treatment than the one in untreated fruit. These results s
uggest thai the increase of firmness of strawberry fruit by 100% CO2 t
reatment may be due to the formation of intramolecular bridges in pect
in, mediated by Ca2-.