NUCLEAR-PROTEIN PATTERNS IN NORMAL T-LYMPHOCYTES AND LYMPHOBLASTOID-CELLS

We compared the two-dimensional patterns of nuclear proteins obtained from normal quiescent T lymphocytes with those from normal proliferati ng T lymphocytes and three lymphoblastoid cell lines (GEM, Namalwa, an d Molt-4). We identified sets of nuclear proteins which are specific f or normal quiescent or normal proliferating T lymphocytes, or shared b y the three lymphoblastoid cell lines and absent from the normal T cel ls, The protein patterns from two nuclear subfractions, i.e., S1 fract ion, obtained after nuclease extraction, and the nuclear matrix, were also analyzed, In S1 nuclear fraction, 6 proteins of 75 kDa [isoelectr ic point (pI) 4.4], 55 kDa (pI 6.7), 41 kDa (pI 4.1), 39 kDa (pI 5.0), 32 kDa (pI 5.5), and 29 kDa (pI 6.6) were found to be specifically pr esent in normal quiescent cells but not in normal proliferating or lym phoblastoid cell lines, Five proteins of 23 kDa (pI 4.2), 23 kDa (pI 4 .3), 22 kDa (pI 4.4), 21 kDa (pI 4.5), and 21 kDa (pI 4.6) were observ ed only in the S1 fraction of normal proliferating lymphocytes, wherea s they were absent in normal quiescent cells and in the transformed ce ll lines. Eight proteins of 56 kDa (pI 4.7), 50 kDa (pI 4.6), 45 kDa ( pI 4.4), 43 kDa (pI 4.3), 42 kDa (pI 4.3), 41 kDa (pI 4.3), 43 kDa (pI 4.2), and 42 kDa (pI 4.1) mere found only in the nuclear matrix of no rmal quiescent cells, Moreover, two doublets of proteins of 31-33 kDa (pI 4.3) and 31-33 kDa (pI 4.2) were found only in the nuclear matrix of the normal proliferating cells and three proteins of 37 kDa (pI 3.8 ), 37 kDa (pI 3.7), and 35 kDa (pI 4.5) were specifically present in t he nuclear matrix of the lymphoblastoid cells lines, but not in normal quiescent or activated lymphocytes.