N. Noguchi et al., CLONING AND NUCLEOTIDE-SEQUENCE OF THE MPHB GENE FOR MACROLIDE 2'-PHOSPHOTRANSFERASE-II IN ESCHERICHIA-COLI, FEMS microbiology letters, 144(2-3), 1996, pp. 197-202
Macrolide 2'-phosphotransferase II [MPH(2')II] inactivates macrolide a
ntibiotics. The mphB gene for MPH(2')II was cloned from Escherichia co
li and sequenced. Analysis of the nucleotide sequence indicated that m
phB encoded a protein of 302 amino acids with a molecular mass of 3448
3 Da. The carboxy-terminal region of the deduced protein contained a s
equence that resembled a conserved functional domain in aminoglycoside
phosphotransferases.