CLONING AND NUCLEOTIDE-SEQUENCE OF THE MPHB GENE FOR MACROLIDE 2'-PHOSPHOTRANSFERASE-II IN ESCHERICHIA-COLI

Authors
NOGUCHI N KATAYAMA J OHARA K
Citation
N. Noguchi et al., CLONING AND NUCLEOTIDE-SEQUENCE OF THE MPHB GENE FOR MACROLIDE 2'-PHOSPHOTRANSFERASE-II IN ESCHERICHIA-COLI, FEMS microbiology letters, 144(2-3), 1996, pp. 197-202
Citations number
26
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
144
Issue
2-3
Year of publication
1996
Pages
197 - 202
Database
ISI
SICI code
0378-1097(1996)144:2-3<197:CANOTM>2.0.ZU;2-6
Abstract
Macrolide 2'-phosphotransferase II [MPH(2')II] inactivates macrolide a ntibiotics. The mphB gene for MPH(2')II was cloned from Escherichia co li and sequenced. Analysis of the nucleotide sequence indicated that m phB encoded a protein of 302 amino acids with a molecular mass of 3448 3 Da. The carboxy-terminal region of the deduced protein contained a s equence that resembled a conserved functional domain in aminoglycoside phosphotransferases.