IDENTIFICATION AND SEQUENCING OF A NOVEL INSERTION-SEQUENCE, IS1471, IN BURKHOLDERIA-CEPACIA STRAIN 2A

A novel insertion sequence (IS), IS1471, has been identified which is inserted into IS element IS1071 possessed by plasmid pIJB1 in Burkhold eria cepacia strain 2a. Nucleotide sequencing has revealed that IS1471 is 1112 bp in length and is flanked by 22/21-bp imperfect inverted re peats with a 3-bp duplication of the target sequence. IS1471 contains a single open reading frame encoding a putative polypeptide of 345 ami no acids with molecular mass of 39 406 Da. Searches of DNA and protein sequence databases did not result in the detection of any homologous IS elements, suggesting that IS1471 is novel and may not belong to any known IS groups.