V. Kyriazopoulou et al., PRENATAL-DIAGNOSIS OF FETAL CYTOMEGALOVIRUS-INFECTION IN SEROPOSITIVEPREGNANT-WOMEN, European journal of obstetrics, gynecology, and reproductive biology, 69(2), 1996, pp. 91-95
Thirty two pregnant women, immune to human cytomegalovirus (HCMV) befo
re conception, were examined with a combination of amniocentesis, and
blood sampling for fetal HCMV infection. The pregnant women had no his
tory of recurrent infection and they all had normal ultrasonographic f
indings. Maternal sera and amniotic fluids were examined for anti-HCMV
antibodies and viral DNA by enzyme immunoassay and polymerase chain r
eaction (PCR). All maternal sera were negative for viral DNA and IgM a
nti-HCMV antibodies. However, HCMV DNA was detected in the amniotic fl
uid of four pregnant women. These four cases were considered as fetal
HCMV infections. Three of them were followed up after birth. The three
neonates appeared clinically normal at birth, but HCMV DNA was detect
ed in their urine specimens and in two of them in the cord blood sera.
Although. the neonates were asymptomatic at birth, the possibility of
developing sequelae later on, cannot be excluded. The results show th
at HCMV recurrent maternal infection is silent and difficult to be sus
pected and diagnosed. They also show that fetal HCMV infection is not
rare among pre-immune pregnant women.