DYSPLASIA EXPRESSES ALTERED NUCLEAR MATRIX PROTEIN-COMPOSITION IN HUMAN ULCERATIVE-COLITIS

We compared nuclear matrix protein compositions (NMPS) in colonic tiss ue from three different patient groups: 1) normal controls; 2) ulcerat ive colitis (UC); and 3) ulcerative colitis with dysplasia (UC-dysplas ia). NMPS were separated by two-dimensional electrophoresis using isoe lectrofocusing and SDS-PAGE. Six specific NMPS (59,500 Kd, pI 6.6 and 6.3; 49,250 Kd, pI 7.4; 33,750 Kd, pI 7.5; 20,000 Kd, pI 7.5; and 19,0 00 Kd, pI 6.3) were identified in UC-dysplasia that were not found in normal controls nor in UC colonic tissue. These observations suggest t hat NMPS may serve as important biochemical markers of dysplasia in UC .