PROPERTIES OF THE REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASETYPE-II FROM HUMAN BRAIN

The regulatory subunit type II (RII) of cAMP-dependent protein kinase purified from human brain was represented by two proteins with apparen t molecular masses of 51-52 kD and 54 kD. Dephosphorylation of human R II containing 3 mol phosphate/mol protein did not change the electroph oretic pattern. One-dimensional peptide mapping of 51-52 kD and 54 kD proteins after digestion with St. aureus V8 protease evidenced to thei r being distinct proteins. The data obtained permit to assume that hum an RII of neural type is represented by two isoforms.