TIME-RESOLVED BIOPHYSICAL METHODS IN THE STUDY OF PROTEIN-FOLDING

Many of the biophysical techniques developed to characterize native pr oteins at equilibrium have now been adapted to the structural and ther modynamic characterization of transient intermediate populations durin g protein folding. Recent advances in these techniques, the use of nov el methods of initiating refolding, and a convergence of theoretical a nd experimental approaches are leading to a detailed understanding of many aspects of the folding process.