THE SEQUENCE OF A 16-691-BP SEGMENT OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-IV IDENTIFIES THE DUN1, PMT1, PMT5, SRP14 AND DPR1 GENES, AND 5 NEW OPEN READING FRAMES

As part of the European BIOTECH programme, the nucleotide sequence of a 16 691 bp fragment from the left arm of chromosome IV of Saccharomyc es cerevisiae has been deduced. Analysis of the sequence reveals the p resence of 13 open reading frames (ORFs) larger than 100 codons. Five of these were previously identified as genes DUN1, PMT1, PMT5, SRP14 a nd DPR1. One putative protein, D2371p, contains an ATP-GTP binding sit e, and shares homology to the ArsA component of an Escherichia coli ar senical pump. No significant homology to any known protein has been fo und for the other ORFs. D2378p contains a zinc finger domain. The nucl eotide sequence has been deposited at EMBL, with Accession Number X956 44.