MULTIVARIATE-ANALYSIS OF CAPILLARY ELECTROPHORESIS SEPARATION CONDITIONS FOR Z-E ISOMERS OF CLOMIPHENE

Plackett-Burman (P-B) experimental design has been used to optimize th e factors affecting separation of Z and E isomers of clomiphene (zuclo miphene and enclomiphene respectively) using capillary electrophoresis . The P-B design was used to simultaneously investigate the following five factors: buffer ionic strength, buffer pH, heptakis (2,3,6-tri-o- methyl) beta-cyclodextrin (TMCD) concentration, methanol concentration and injection time, each at three levels. In addition to these, a dum my variable was added to estimate the variability of the system. Effec ts on resolution and analysis time were calculated. Based on the infor mation gained from the P-B design, the following set of conditions was chosen: 100 mM phosphate buffer pH 2.3, 5 mM TMCD, 5% methanol, and 1 .7 s hydrodynamic injection time. These conditions gave well-resolved peaks for zuclomiphene and enclomiphene.