RECOVERY OF EXTRACELLULAR INULINASE BY EXPANDED BED ADSORPTION

Authors
PESSOA A HARTMANN R VITOLO M HUSTEDT H
Citation
A. Pessoa et al., RECOVERY OF EXTRACELLULAR INULINASE BY EXPANDED BED ADSORPTION, Journal of biotechnology, 51(1), 1996, pp. 89-95
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of biotechnology → ACNP
ISSN journal
01681656
Volume
51
Issue
1
Year of publication
1996
Pages
89 - 95
Database
ISI
SICI code
0168-1656(1996)51:1<89:ROEIBE>2.0.ZU;2-I
Abstract
Adsorption of inulinase onto STREAMLINE (TM) ion-exchange adsorbents i n the absence and presence of Candida kefyr cells has been studied. Th e favourable adsorption pH was in the range of 6.5 and 7.0 using the w eak anion exchanger (DEAE). In the case of the strong cation exchanger (SP), pH 4.0 was found to be appropriate. The adsorption isotherms fi tted to the Langmuir equation well. Expanded bed adsorption performed with original cultivation medium showed enzyme recovery of 93%. The en richment and the concentration factors were 5.8 and 2.8, respectively. The presence of cells did not cause problems.