PARASIGHT(TM)-F TEST FOR THE DETECTION OF TREATMENT FAILURE IN MULTIDRUG-RESISTANT PLASMODIUM-FALCIPARUM MALARIA

The ParaSight(TM)-F test for the detection of Plasmodium falciparum wa s evaluated for its accuracy and usefulness in predicting treatment ou tcome in 75 patients (70 males, 5 females) with acute uncomplicated ma laria who attended a malaria clinic in Mae Set, Tak province, on the T hai-Myanmar border. All patients were admitted to the clinic for 28 d to exclude reinfection. The test was performed using blood samples col lected into ethylenediaminetetraacetic acid from the patients on admis sion, and on days 1, 2, 7, and 14. The presence of microscopically det ectable parasitaemia was used as the reference for sensitivity and spe cificity of the rest. The reappearance of parasites on day 28 was used to determine the accuracy of predicting the outcome of artemether tre atment on day 14. The sensitivity of the ParaSight(TM)-F test on admis sion, and on days 1, 2, 7, and 14, was 98.7%, 96.7%, 100%, 100% and 10 0%, respectively, with corresponding specificities of 50%, 24.2%, 47.1 % and 72.9%. The sensitivity for predicting recrudescence by using the test on day 14 was 100%, with 97.7% specificity, and the sensitivity of predicting a sensitive response on day 14 was 97.7%, with 100% spec ificity. The test seems to permit more precise detection of treatment failure under 'field' conditions if used on day 14 after the start of treatment.