SEPARATION OF PHOSPHOLIPID DERIVATIVES BY MICROCOLUMN LIQUID-CHROMATOGRAPHY

Packed, fused silica capillaries were employed, at high chromatographi c resolution, to separate complex mixtures of phospholipids derived fr om plant and animal sources. Benzoylation of phosphatidylcholine deriv atives and dansylation of phosphatidylethanolamines were used in conju nction with miniaturized spectroscopic detectors. Under the conditions of stepwise gradient elution, detection limits for both derivatives w ere on the order of picomoles. (C) 1996 John Wiley & Sons, Inc.