Cm. Lee et al., CONTIG MAPPING OF ARABIDOPSIS-THALIANA CHROMOSOME-1 ENCOMPASSING AP-1AND CLV-1 BY USING YEAST ARTIFICIAL CHROMOSOME, Molecules and cells, 6(5), 1996, pp. 557-562
By using yeast artifical chromosome (YAC) clones, we have generated a
contig map of the long arm of the Arabidopsis thaliana chromosome numb
er 1. Using the six chromosome 1-specific RFLP markers, seventeen YAC
clones were identified, and the end clones of these 17 YAC clones were
isolated for further chromosome walking. At least two YAC clones have
been identified for each of the RFLP markers used. The YACs encompass
at least 27.2 cM genetic distances in the RFLP map between the RFLP m
arkers, 2488-B and 17311, which cover 3,800 kb in chromosome 1 of the
genome. The YACs we identified also include two Arabidopsis mutant gen
es, ap-1 and clv-1.