RENAL-CELL CARCINOMA-SPECIFIC LYSIS BY CYTOTOXIC T-LYMPHOCYTE CLONES ISOLATED FROM PERIPHERAL-BLOOD LYMPHOCYTES AND TUMOR-INFILTRATING LYMPHOCYTES

Melanoma and renal-cell carcinoma (RCC) are generally considered to be relatively immunogenic tumor types in humans. In the case of melanoma , many major histocompatibility complex (MHC) class I-restricted tumor -specific cytotoxic T lymphocytes (CTL) have been isolated from either tumorinfiltrating lymphocytes (TIL) or autologous peripheral blood ly mphocytes (PBL). In contrast, such CTL have only incidentally been des cribed in the case of RCC. It has often been reported that TIL lines i solated from RCC display non-MHC-restricted and non-specific activity. Here, we report the isolation and characterization of tumor-specific CTL from PBL of one RCC patient and from TIL of another RCC patient. C TL clones 263/17 and 263/45, isolated from the PBL of patient LE-9211, were restricted by HLA-B7. CTL clone 5E, isolated from the TIL of pat ient LE-8915, was restricted by HLA-B37. The autologous RCC cell lines were efficiently lysed by the CTL clones, whereas normal epithelial c ells of the proximal tubuli matched for the restriction element and K5 62 were not. From a panel of allogeneic RCC cell lines, CTL 5E recogni zed MZ-1940-RCC. Reactivity to allogeneic RCC sharing HLA-B7 was also observed with CTL 263/17 and 263/45, both of which could lyse the HLA- B7-positive cell line MZ-1851-RCC. Our data provide evidence that comm on tumor antigens are recognized by CTL on RCC. (C) 1996 Wiley-Liss, I nc.