HPLC AND NINHYDRIN PHOTOMETRIC-DETERMINATION OF AMINO-ACIDS IN CACO3 RICH LACUSTRINE SEDIMENTS OF THE JURA REGION, FRANCE

Citation
R. Moilleron et al., HPLC AND NINHYDRIN PHOTOMETRIC-DETERMINATION OF AMINO-ACIDS IN CACO3 RICH LACUSTRINE SEDIMENTS OF THE JURA REGION, FRANCE, Annales de Limnologie, 32(3), 1996, pp. 197-204
Citations number
15
Categorie Soggetti
Limnology
Journal title
ISSN journal
00034088
Volume
32
Issue
3
Year of publication
1996
Pages
197 - 204
Database
ISI
SICI code
0003-4088(1996)32:3<197:HANPOA>2.0.ZU;2-5
Abstract
A study]las been carried out on the acid soluble fraction (hydrosysis at pH 1.5, 3N HCl, 20 % KCl) of organic matter (OM) in the highly calc areous lacustrine sediments of the Jura region, France. Amino acids (A A's) were quantified and determined ninhydrin photometry and HPLC resp ectively. There was a high degree of interference by calcium in both p rocedures. The mechanism of interference is explained and methodology adopted to overcome it, by removal of calcium, are dealt with. The res ults of both methods are compared.