ISOLATION OF HANDELIN FROM CHRYSANTHEMUM-BOREALE

The flowers of Chrysanthemum boreale afforded handelin, a unique guaia nolide dimer and a mixture of n-hydrocarbons and n-hydrocarbon alcohol s in addition to beta-sitosterol and beta-sitosterol glucoside. Detail ed analysis of the H-1- and C-13-NMR spectra of handelin was carried o ut by the application of two-dimensional H-1- H-1-COSY and H-1- C-13 m ultiple-bond, multiple-quantum spectroscopic correlation techniques. H andelin was inactive in the in vitro anti-tumor activity.