MODIFIED NUCLEOTIDES AS SUBSTRATES AND INHIBITORS OF ADENYLATE KINASEFROM DIFFERENT SOURCES

The substrate and inhibitory properties of modified nucleotides with r espect to adenylate kinase from rabbit muscles, human placenta and Esc herichia coli were studied. A number of 5'-hydrogenphosphonates and 5' -fluorophosphates of modified nucleotides were shown to inhibit the ph osphorylation reaction catalyzed by these enzymes. A clear difference between phosphonates of 3'-deoxyribonucleotides and the corresponding ribo- and 2',3'-dideoxyribonucleotides was found. 3'-Azido-2',3'-dideo xythymidine and its phosphorus derivatives did not inhibit the adenyla te kinase reaction.