IN-VIVO MODELS OF THROMBOSIS FOR THE ANTIPHOSPHOLIPID SYNDROME

Utilizing this unique animal model of thrombosis we demonstrated that human (IgG, IgM or IgA) polyclonal and monoclonal antiphospholipid ant ibodies derived from APS patients have a significant enhancing effect on thrombus formation. This effect is reversed by treatment of the mic e with hydroxychloroquine (plaquenil). In addition murine polyclonal a nd monoclonal anticardiolipin antibodies induced by active immunizatio n with human beta(2)-GP1 or human anticardiolipin antibodies showed to have thrombogenic properties in CD1 mice. Antibodies with antihuman b eta(2)-GP1 activity alone did not seem to affect thrombus formation.