Ad. Kligerman et al., CYTOGENETIC EFFECTS OF BUTADIENE METABOLITES IN RAT AND MOUSE SPLENOCYTES FOLLOWING IN-VITRO EXPOSURES, Toxicology, 113(1-3), 1996, pp. 336-340
As a first step in investigating the genotoxic effects of the principa
l metabolites of 1,3-butadiene (ED) in both rats and mice, splenocytes
(which have little mixed function oxidase activity) from each specime
n were exposed to a series of concentrations of either 3,4-epoxy-1-but
ene (EB) (20 to 931 mu M) or 1,2:3,4-diepoxybutane (DEB) (2.5 to 160 m
u M) for 1 h. The splenocytes were then washed, cultured, and stimulat
ed to divide with concanavalin A, and metaphases were analyzed for the
induction of sister chromatid exchanges (SCEs) and chromosome aberrat
ions (CAs). In addition, cells from some experiments were taken after
exposure but before culture, and subjected to the single cell gel (SCG
) assay to measure DNA damage in the form of DNA strand breakage and/o
r alkaline-labile sites. Initial studies indicate that EB does not ind
uce cytogenetic damage in either rat or mouse G(0) splenocytes. Howeve
r, DEB was an extremely potent SCE- and CA-inducer in both species wit
h no species differences apparent. Neither DEB nor EB produced any sta
tistically significant DNA-damaging effects as measured by the SCG ass
ay.