CHROMOSOME PAINTING ANALYSIS OF SPONTANEOUS AND METHYL METHANESULFONATE-INDUCED TRIFLUOROTHYMIDINE-RESISTANT L5178Y CELL COLONIES

Citation
Ls. Zhang et al., CHROMOSOME PAINTING ANALYSIS OF SPONTANEOUS AND METHYL METHANESULFONATE-INDUCED TRIFLUOROTHYMIDINE-RESISTANT L5178Y CELL COLONIES, Mutation research. Genetic toxicology testing, 370(3-4), 1996, pp. 181-190
Citations number
33
Categorie Soggetti
Genetics & Heredity",Toxicology
ISSN journal
01651218
Volume
370
Issue
3-4
Year of publication
1996
Pages
181 - 190
Database
ISI
SICI code
0165-1218(1996)370:3-4<181:CPAOSA>2.0.ZU;2-I
Abstract
Spontaneous and methyl methanesulfonate-induced trifluorothymidine-res istant mutants in mouse lymphoma L5178Y cells were analyzed using fluo rescence in situ hybridization with mouse probes specific for chromoso me 11, on which the tk gene is located, and chromosome 3, as the contr ol. 76.5% (13/17) of small-colony mutants (thought to be the result of chromosomal mutation) and 28.6% (4/14) of large-colony mutants (thoug ht to be the result of gene mutation) showed rearranged chromosome 11. Of the mutants with abnormal chromosome 11 painting pattern, 5 small- and 2 large-colony mutants carried clonal aberrations, while the rema ining 8 small- and 2 large-colony mutants showed mosaic aberrations. M ost abnormalities in the small-colony mutants involved the distal regi on of one painted chromosome 11, where the tk(+) gene maps. An increas e, rather than a decrease, in chromosome 11 material was found in a ma jority of abnormally painted mutants. On the contrary, no rearrangemen ts involving chromosome 3 were found in any small- and large-colony mu tants analyzed except one large-colony mutant, which showed chromosome rearrangements involving both chromosome 11 and 3. The present study confirms that the majority of small-colony mutants in L5178Y cells hav e chromosome 11 rearrangements that can be detected by chromosome pain ting and that the majority of the chromosomal abnormalities in TFT-res istant mutants involved complex rearrangements.