LACK OF INDUCTION OF CHROMOSOME-ABERRATIONS AND MICRONUCLEI BY N-(1,3-DIMETHYLBUTYL)-N-PHENYLPARAPHENYLENEDIAMINE IN MOUSE BONE-MARROW CELLS

N-(1,3-Dimethylbutyl)-N-phenylparaphenylenediamine (DMPPD) is a deriva tive of phenylenediamine (PPD) which is widely used in the rubber indu stry as an antioxidant. DMPPD which is a strong allergen, is least stu died for its clastogenic potency. This study evaluated the genotoxic p roperty of DMPPD in Swiss albino mice bone marrow cells by using chrom osomal aberration and micronuclei assay. Three concentrations of DMPPD , i.e., 100, 150 and 200 mg/kg body wt. did not significantly increase the micronuclei in polychromatic erythrocytes or the ratio of poly to normochromatic erythrocytes. Chromosome aberration studies using 100 mg and 200 mg/kg body wt. showed that DMPPD is a non-inducer of chromo some aberrations. The results indicated non-clastogenicity of DMPPD in mice marrow cells up to a concentration of 200 mg/kg body wt. under o ur experimental conditions.