J. George et R. Kuttan, LACK OF INDUCTION OF CHROMOSOME-ABERRATIONS AND MICRONUCLEI BY N-(1,3-DIMETHYLBUTYL)-N-PHENYLPARAPHENYLENEDIAMINE IN MOUSE BONE-MARROW CELLS, Mutation research. Genetic toxicology testing, 370(3-4), 1996, pp. 191-194
N-(1,3-Dimethylbutyl)-N-phenylparaphenylenediamine (DMPPD) is a deriva
tive of phenylenediamine (PPD) which is widely used in the rubber indu
stry as an antioxidant. DMPPD which is a strong allergen, is least stu
died for its clastogenic potency. This study evaluated the genotoxic p
roperty of DMPPD in Swiss albino mice bone marrow cells by using chrom
osomal aberration and micronuclei assay. Three concentrations of DMPPD
, i.e., 100, 150 and 200 mg/kg body wt. did not significantly increase
the micronuclei in polychromatic erythrocytes or the ratio of poly to
normochromatic erythrocytes. Chromosome aberration studies using 100
mg and 200 mg/kg body wt. showed that DMPPD is a non-inducer of chromo
some aberrations. The results indicated non-clastogenicity of DMPPD in
mice marrow cells up to a concentration of 200 mg/kg body wt. under o
ur experimental conditions.