DETERMINATION OF FLUDARABINE PHOSPHATE IN HUMAN PLASMA USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
MISZTAL G PAW B
Citation
G. Misztal et B. Paw, DETERMINATION OF FLUDARABINE PHOSPHATE IN HUMAN PLASMA USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Die Pharmazie, 51(10), 1996, pp. 733-734
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
Die Pharmazie → ACNP
ISSN journal
00317144
Volume
51
Issue
10
Year of publication
1996
Pages
733 - 734
Database
ISI
SICI code
0031-7144(1996)51:10<733:DOFPIH>2.0.ZU;2-6
Abstract
A rapid, simple and accurate HPLC method for the determination of flud arabine phosphate in human plasma is presented. Fludarabine phosphate from plasma was successfully purified. The samples were chromatographe d on a LiChrosorb RP-18 (10 mu m) column after purification using a Ba kerbond Spe column. The mobile phase was methanol/phosphoric buffer pH 4.15 (20:80). The detection was carried out at 262 nm. The method was tested for linearity (range from 100 to 900 ng/ml), recovery (ca. 99. 8%) and precision (C.V. = 3.2%).