SEPARATION OF ENZYMES FROM POLYENZYME MIXTURES USED IN MEDICINE AND PHARMACY .1. ISOLATION AND CHARACTERIZATION OF XYLANASE FROM ASPERGILLUS-ORYZAE

Three multiple forms (F-I, F-II and F-III) of xylanase produced from A spergillus oryzae were established in the commercial preparation Luizy m(R). Two methods were used (ion-exchange chromatography and polyacryl amide gel electrophoresis). The active xylanase fraction (F-III-II-I) was purified and characterized (pH(opt) 4.5; T-opt 37 degrees C and mo lecular mass 38.5 kDa). The products of the enzymatic reaction were an alysed by HPLC. The hydrolysis pattern showed that the xylanase was an exo-endo acting enzyme.