L. Kubala et al., DETERMINATION OF PHAGOCYTE ACTIVITY IN WH OLE-BLOOD OF CARP (CYPRINUS-CARPIO) BY LUMINOL-ENHANCED CHEMILUMINESCENCE, Veterinarni medicina, 41(10), 1996, pp. 323-327
In this study, the phagocytic capacity of fish peripheral blood leucoc
ytes was studied using the luminol-enhanced chemiluminescence (CL) met
hod. Suspensions of rice starch (1%) or opsonized zymosan particles -
OZP (0.2%) were used as activators. The level of CL was detected at 25
degrees C in various whole blood volumes (100, 50, 20 a 10 mu l) duri
ng the mixed or unmixed measurement mode. The peak of CL in all starch
-activated samples was between 2.0-4.0 mV, regardless of blood volume
or a mode of measurement. Similar results were obtained for OZP activa
ted CL when measured in the mixed mode. On the other hand, the peak of
OZP activated CL in unmixed samples was much higher and it showed pos
itive correlation with the increasing volume of blood in a sample. In
contrast, no significant differences between the reaction curve areas
were found for different blood volumes. After correction for phagocyte
number the highest CL activity per cell was observed in the samples c
ontaining 10 mu l of blood. It can be concluded that 10-20 mu l of blo
od are optimal volumes for the measurements of respiratory burst activ
ity in the whole blood of carp and opsonized zymosan particles should
be used as an activator. At the same time, the unmixed mode of measure
ment seems to give better results than the mixed one.