A NEW VERSION OF AN IN-SITU SAMPLING SYSTEM FOR BIOPROCESS ANALYSIS

In this article, attention is focussed on an on-line sampling device f or bioreactors and its characterization regarding sterility and respon se time. The integration of this device into analytical systems for bi otechnology (FIA with biosensors) resulted in on-line analysis systems for bioprocess control. The new ESIP version of an in situ sampling s ystem for bioprocess analysis produced by the EPPENDORF-NETHELER-HINZ GmbH, Germany, had a short response time of 8 min (99%), which was det ermined by means of conductivity measurement after an increase of the medium conductivity due to the gradual addition of KCl. Effectiveness and reliability of the module were tested by bubble point measurement resulting in a bubble point pressure of 2.1 bars. The sampling probe w as tested successfully for use in a broad variety of microorganisms an d cultivations.