CULTURE OF CELLS DERIVED FROM THE HUMAN SEBACEOUS GLAND UNDER SERUM-FREE CONDITIONS WITHOUT A BIOLOGICAL FEEDER LAYER OR SPECIFIC MATRICES

We succeeded in serially culturing cells derived from human sebaceous gland (sebocytes) under serum-free conditions. Sebaceous glands were i solated from dispase-treated facial skin specimens and cultured using two different methods, explant culture and dispersed cell culture, in KGM. In both types of culture the sebocytes proliferated rapidly witho ut a biological feeder layer or specific matrices, It was possible to cultivate the cells serially for at least three passages (explant cult ure) and six passages (dispersed cell culture), and to store the cells in liquid nitrogen with good recovery, Analytical thin-layer chromato graphy revealed that the cells synthesized a large amount of sebum-spe cific lipids, squalene and wax esters in vitro. Both testosterone and Sa-dihydrotestosterone significantly stimulated the proliferation of t he sebocytes, The methods presented here may permit a large series of studies on the function of sebaceous glands or cells derived from seba ceous glands.