DETECTION AND SEQUENCE-ANALYSIS OF THE MAJOR IMMEDIATE-EARLY AND PP150 GENE OF LATENT HUMAN CYTOMEGALOVIRUS IN SPLEEN, LIVER, AND KIDNEY TISSUES OF TRAUMA VICTIMS
Mgr. Hendrix et al., DETECTION AND SEQUENCE-ANALYSIS OF THE MAJOR IMMEDIATE-EARLY AND PP150 GENE OF LATENT HUMAN CYTOMEGALOVIRUS IN SPLEEN, LIVER, AND KIDNEY TISSUES OF TRAUMA VICTIMS, Journal of medical virology, 50(2), 1996, pp. 193-197
The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and
kidney samples of HCMV-seropositive trauma victims during latency was
demonstrated by polymerase chain reaction (PCR), using primers reacti
ve with the major immediate early gene exon 4 and the structural gene
pp150. Sequence analysis of the PCR amplificates showed more than 95%
homology with the reference HCMV strain AD169. The few mutations obser
ved were mostly distributed randomly. In one subject two types of the
MIE-4 gene were detected, and in another subject two types of the pp15
0 gene were found, suggesting that different strains of HCMV can be fo
und in organs of the same patient during latency. (C) 1996 Wiley-Liss,
Inc.