AGE-DEPENDENT VARIATIONS OF LACTATE-DEHYDROGENASE AND CREATINE-KINASEACTIVITIES IN WATER-BUFFALO CALF SERUM

The electrophoretic patterns of the serum enzymes lactate dehydrogenas e and creatine kinase from water buffalo calves are described. Differe nces in total activities as well as their relative distribution were s een at ages ranging from 1 to 10 weeks. While total lactate dehydrogen ase activity increased by over 100%, total creatine kinase increased b y almost 400%. The relative activities of lactate dehydrogenase 1 and 5 decreased with age. Lactate dehydrogenase 2 and 3 increased and lact ate dehydrogenase 4 did not change. In relation to creatine kinase, th e prevalent isoenzyme was creatine kinase-MM, but it's relative activi ty gradually decreased in comparison to the other two isoenzymes (crea tine kinase-MB and creatine kinase-BB). Creatine kinase-BB was complet ely absent until the 3rd week of age. The percentage modifications of creatine kinase isoenzymes were correlated to age. The results suggest that isoenzymatic separation and characterization of lactate dehydrog enase and creatine kinase in relation to the various tissues can signi ficantly contribute to the diagnosis of diseases which are linked to t issue damage.