EFFECTS OF METHYLGLYOXAL BIS(CYCLOPENTYLAMIDINOHYDRAZONE) (MGBCP) ON CELL-CYCLE PROGRESSION IN 3 HUMAN OSTEOSARCOMA CELL-LINES

Our previous experiments have indicated that the antitumor effects of a polyamine biosynthetic inhibitor, methylglyoxal bis(cyclopentylamidi nohydrazone) (MGBCP), on human osteosarcoma cell lines such as MG-63, G-292 and HOS cells are obtained by its action depleting the cellular polyamine contents. In the present study, the effects of polyamine dep letion by MGBCP on the cell cycle progression in these osteosarcoma ce ll lines were investigated by flow cytofluorometric analysis. MGBCP ar rested the tumor cells at the G1 phase by preventing the G/S phase tra nsition. Mitotic indexes (MIs) in these MGBCP-inhibited tumor cells we re also decreased. These findings suggest that MGBCP suppresses the ce ll cycle progression in osteosarcoma MG-63, G-292 and HOS cells by inh ibiting intracellular polyamine biosynthesis.