EXPRESSION PATTERN OF NUCLEAR P53 IS UNIQUE IN COS-1 CELLS

As p53 is transcriptionally inactive in COS-1 cells, we felt it would be interesting to study the relative time course of expression of nucl ear p53 and the onset of S phase. Our results indicated that the expre ssion of nuclear p53 lagged behind the onset of S phase in COS-1 cells . In addition, rapid nuclear expression of p53, probably resulting fro m nuclear translocation, occurred immediately after the addition of se rum into COS1 cells. No such phenomena were found in B104-1 and BHK ce lls.